A fixed dose of Valacyclovir is used in tablet dosage form for the treatment and prevention of infectious diseases caused by Herpes Zoster. A simple, precise and accurate HPTLC method was developed and validated for the determination of Valacyclovir in Tablet dosage form. Separation was obtained using Pre-coated silica gel aluminum plate 60 F254 TLC plate with mobile phase Ethanol:Ethyl acetate:Ammonia (7.5:2.5:0.6) in which spot was devoid of tailing and sharp with good Rf value of 0.68±0.02 at 254 nm in remission/extinction mode with CD60 TLC scanner using ProQuant software. The method was validated for linearity, precision, accuracy, solution stability and specificity. The accuracy and reliability of the method was assessed by evaluation of linearity (500-3000 ng/band), precision (intra-day % RSD 0.87-1.19 and inter-day % RSD 0.90-1.34, accuracy (%Recovery) (98.89-101.98 %) and specificity, in accordance with ICH guidelines. Limit of detection and limit of quantification was found to be 23.29 ng/band and 70.59 ng/band.
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